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. 2013 Mar 19;8(3):e59057. doi: 10.1371/journal.pone.0059057

Figure 4. Overexpression of miR-33a, miR-149 and miR-193a-3p mimic reduced expression levels of predicted target proteins in WRO cells.

Figure 4

(A) Transfection of miR mimics (33a-m, 149-m and 193a-m) significantly increased respective miRNA expression, whereas control miR mimic (cont-m) had no such effect, as quantified by real-time quantitative RT-PCR. (B) Western blotting shows that transfection of miR mimic did not change XB130 protein levels. (C–E) MYC, FOSL1 and SCL7A5 are predicted target of miR-33a, miR-149 and miR-193-3p, respectively. Upper panel shows examples that the expression of the predicted target protein was decreased by respective miR mimic transfections as determined by Western blotting. Lower panel shows the quantification of the expression levels by densitometry.