Table 1. Pilot SLAF-seq data summary in rice and soybeans.
Genome information | Rice | Soybeans |
Genome size (Mb) | 382.79 | 950.07 |
% of repeats in genome | 39.11% | 42.96% |
GC content | 43.56% | 34.67% |
Expected information | ||
Enzymes | HaeIII+MseI | HaeIII+MseI |
Expected SLAF size range (bp) | 384–454 | 369–439 |
Expected no. of SLAFs | 21,074 | 76,970 |
SLAF density per 100 kb | 5.51 | 8.10 |
Simplification ratio | 0.33% | 0.49% |
% of SLAFs in repeats | 17.78% | 25.32% |
Observed information | ||
Total reads | 546,271 | 1,341,599 |
Observed no. of SLAFs | 25,433 | 83,055 |
Matched no. of SLAFs | 19,005 | 61,056 |
Matched % of SLAFs | 90.18% | 79.32% |
Reads % in matched SLAFs | 82.48% | 65.86% |
SLAF average depth | 18.95 | 12.11 |
SLAFs per 100 kb | 6.64 | 8.74 |
Simplification ratio | 0.40% | 0.52% |
% of SLAFs in repeats | 19.49% | 25.34% |