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Reverse transcription polymerase chain reaction for corticotropin-releasing factor 2 receptor gene expression in total RNA isolated from the human colon cancer cell line Caco2 (1), human colon (2), and human brain (3) from healthy subjects. The predicted size products (308 bp) were amplified in the human colon and brain samples. Negative controls in the absence of reverse transcription enzyme (4) or DNA template (5) are also presented. CRF: Corticotropin-releasing factor; M: Marker.
