Figure 5. Role of RKIP in cervical cancer cells response to cisplatin.

A) Representative pictures of the nonlinear regression analysis of cervical cancer cell lines treated with cisplatin for determination of half maximal inhibitory concentrations (IC₅₀). B) Graphic representation of the mean IC₅₀ values for cisplatin in the cervical cancer cell lines. The transfected cells with shRKIP were less sensitive to cisplatin treatment in the three different cell lines. C) HeLa transfected cell lines were exposed to increasing concentrations of cisplatin by 24 hours. Cisplatin treatment induced ERK activation (p-ERK1/2) and apoptosis of the cells as evaluated by PARP (total and cleaded specific antibodies) and caspase-9 cleavage, mainly in the empty vector transfected cells. All the experiments were done in triplicate at least three times. Data in the panels A and B are represented as the mean ± SD.