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. 2013 Mar 19;8(3):e58776. doi: 10.1371/journal.pone.0058776

Figure 5. Anterograde spread capacity of Us9 acidic cluster point mutants.

Figure 5

Viral titers in the S (left panel) and N (right panel) compartment of chambered SCG cultures 24 hours post-infection with: Untagged Us9: PRV Becker (wild-type), PRV 171 (Us9 E52A D54A N55A E56A), PRV 173 (Us9 S51,53A). GFP-tagged Us9: PRV 340 (GFP-Us9), PRV 451 (GFP-Us9 S51,53A), PRV 452 (GFP-Us9 S51A), and PRV 453 (GFP-Us9 S53A). Point estimates reflect viral titers in each compartment, representative of 2 biological replicates, each performed in triplicate. Line denotes median value.