Figure 7. Phosphorylation of Us9 is not essential for Kif1A binding.

Differentiated PC12 cells were infected with the indicated PRV strains, lysed at 12 hours post-infection, and subject to co-immunoprecipitation analysis using anti-GFP rabbit polyclonal antibodies. The ability of these mutant GFP-Us9 variants to interact with KIf1A was specifically assessed through WB detection.