Fig. 4.

Effects of rice bran extracts on the MUC2 gene in LS174T cells. Cells (2 × 10⁴) were precultured in DMEM for 12 h, and subsequently incubated alone (), or with 0.1 mg/ml of RBWE () or 1.0 mM sodium butyrate () for 4 days. Total RNA was isolated, and the mRNA expression was analyzed with real-time RT-PCR. The data are expressed relative to values for untreated control cells and represent the mean ± SD (n = 3, *p < 0.05, **p < 0.01)