Table 2.
AA | Temperatures/°C | |||||
---|---|---|---|---|---|---|
55 | 65 | 75 | 85 | 95 | 100 | |
Asp | 60.5 ± 1.31 d | 80.3 ± 5.02 cd | 131.6 ± 3.18b | 98.1 ± 4.33c | 197.4 ± 2.69a | 120.4 ± 8.94b |
Ser | 48.5 ± 2.26c | 66.3 ± 3.15a | 54.7 ± 2.99bc | 59.8 ± 1.86b | 52.6 ± 2.11bc | 28.5 ± 0.08 d |
Glu | 209.1 ± 7.01a | 189.0 ± 8.43b | 181.7 ± 6.86b | 180.3 ± 5.83b | 141.1 ± 4.26c | 72.7 ± 1.32 d |
Gly | 107.6 ± 1.04c | 111.3 ± 1.95c | 128.7 ± 2.88b | 138.8 ± 4.09a | 122.5 ± 6.45b | 107.6 ± 2.87c |
His | 33.4 ± 0.06b | 19.3 ± 0.11 d | 43.3 ± 0.37ab | 48.3 ± 1.02a | 34.4 ± 0.06b | 29.8 ± 0.15c |
Arg | 523.7 ± 8.93c | 648.33 ± 4.76b | 810.7 ± 7.11a | 884.9 ± 9.93a | 839.9 ± 11.97a | 693.3 ± 4.98b |
Thr | 30.4 ± 1.34bc | 45.30 ± 1.08a | 35.3 ± 1.00b | 29.3 ± 0.02bc | 21.4 ± 0.01c | 16.9 ± 0.31 d |
Ala | 402.8 ± 3.55a | 422.7 ± 9.83a | 291.8 ± 7.16c | 336.0 ± 6.81b | 264.1 ± 1.06c | 138.8 ± 3.84 d |
Pro | 27.8 ± 1.76c | 38.5 ± 1.20ab | 34.3 ± 0.85b | 46.1 ± 0.16a | 44.8 ± 3.76a | 25.9 ± 1.07c |
Cys2 | 1.7 ± 0.06 cd | 1.3 ± 0.01 cd | 13.5 ± 0.12b | 31.1 ± 0.64a | 5.2 ± 1.07c | 0.89 ± 0.03 d |
Tyr | 25.0 ± 0.5bc | 35.5 ± 0.02a | 24.5 ± 1.05bc | 30.7 ± 0.67b | 22.4 ± 1.63c | 11.3 ± 4.42 d |
Val | 25.6 ± 2.89c | 47.4 ± 6.07b | 47.1 ± 5.32b | 71.7 ± 3.88a | 48.5 ± 1.46b | 26.4 ± 5.00c |
Met | 40.3 ± 0.73c | 82.3 ± 2.89b | 102.3 ± 4.93a | 108.5 ± 5.91a | 31.6 ± 6.11 d | 10.3 ± 0.22e |
Lys | 37.8 ± 0.79 d | 49.6 ± 3.30 cd | 71.7 ± 2.58b | 75.4 ± 1.16ab | 86.6 ± 5.20a | 58.3 ± 2.04c |
Ile | 27.6 ± 0.12 cd | 22.8 ± 2.06 d | 33.9 ± 2.21c | 51.6 ± 3.04a | 43.7 ± 4.11b | 18.5 ± 0.97 d |
Leu | 50.1 ± 2.07b | 63.2 ± 1.99a | 53.8 ± 3.75b | 66.8 ± 2.00a | 55.3 ± 2.98b | 26.9 ± 0.01c |
Phe | 36.5 ± 3.95c | 26.1 ± 4.00 d | 52.8 ± 0.91b | 67.0 ± 1.57a | 23.7 ± 3.97 d | 15.8 ± 0.01e |
∑UTAA | 816.6 ± 4.09a | 829.3 ± 2.01a | 786.5 ± 3.01b | 831.0 ± 1.25a | 748.7 ± 3.08c | 455.3 ± 2.15 d |
∑BCAAs | 103.4 ± 1.05c | 133.4 ± 2.81bc | 134.8 ± 1.32bc | 190.0 ± 1.18a | 147.6 ± 0.97b | 71.9 ± 0.54 d |
∑FAA | 1688.7 ± 9.07 d | 1949.1 ± 7.12c | 2111.6 ± 3.99b | 2335.1 ± 5.80a | 2035.1 ± 9.67bc | 1402.4 ± 5.96 d |
Means followed by different letters in a row are significantly different (P ≤ 0.05) (n = 3). FAA free amino acids, UTAA Unami-Taste amino acids, BCAAs Branched-Chain Amino Acids