Figure 4. Selection and confirmation of positives. A).

Scatter plot analysis of the average percentage inhibition (%I) of each compound toward Y79 cells, in absence of resveratrol (−RES) or with 80 µM resveratrol co-treatment (+RES). Initial positives with agonistic activity are selected as those compounds inducing greater than or equal to 50% inhibition in presence of resveratrol and inducing less than or equal to 50% inhibition in absence of resveratrol (green). Initial positives with antagonistic activity are selected as those compounds inducing less than or equal to 50% inhibition in presence of resveratrol and inducing greater than or equal to 50% inhibition in absence of resveratrol (red). B) Scatter plot analysis of the average percentage inhibition (%I) of each compound toward RB355 cells, in absence of resveratrol (−RES) or with 80 µM resveratrol co-treatment (+RES). Positives from the Y79 screen with agonistic activity in both screens are selected as those compounds inducing greater than or equal to 50% inhibition in presence of resveratrol and inducing less than or equal to 50% inhibition in absence of resveratrol (green). Positives from the Y79 screen with antagonistic activity in both screens are selected as those compounds inducing less than or equal to 50% inhibition in presence of resveratrol and inducing greater than or equal to 50% inhibition in absence of resveratrol (red). C) Heatmap representation of the IC₅₀ values of the selected 21 positives (8 agonists and 13 antagonists) toward Y79 and RB355 cells in absence of resveratrol or with 30 or 80 µM resveratrol co-treatment. The triangle symbolizes the increasing concentration in resveratrol.