. 2013 Mar 19;8(3):e59156. doi: 10.1371/journal.pone.0059156

Table 1. Summary of IC₅₀ in the Alamar Blue viability assay readout for eight resupplied agonists and 13 antagonists toward the Y79 and RB355 human retinoblastoma cell lines.

			Alamar Blue viability assay IC₅₀ (µM)
			Y79			RB355
	Compound name	Biological activity	0 µM resv.	30 µMresv.	80 µMresv.	0 µMresv.	30 µMresv.	80 µMresv.
Agonist	Berberine chloride	Antibiotic	24*	15	8.6	59*	23	6.8
	Perphenazine	Antipsychotic	32	27	16	25	19	9.8
	Avermectin B1	Antiparasitic	>100*	>100	>100	55*	47*	60*
	Phorbol 12-myristate 13 acetate	PKC activator	>100	>100	>100*	>100	>100	67*
	Budesonide	Steroid	>100	>100	>100*	>100	>100	>100*
	Piperazine carbazole ethanol derivative	Cyto C release inhibitor	9.2	7.9	7.4	12	8.7	7.0
	Triamterene	Diuretic	>100*	>100*	>100*	33*	>100*	>100*
	Lercanidipine	Ion channel inhibitor	38*	36*	38*	19	57*	33*
Antagonist	Nocodazole	Microtubule inhibitor	34*	21*	5.6*	1.1	0.53*	>100*
	Chelidonine	Microtubule inhibitor	6.8*	7.9*	4.9*	1.6	1.3*	>100*
	Albendazole	Microtubule inhibitor	>100*	>100*	>100*	20*	23*	>100*
	ZM 447439	Aurora B kinase inhibitor	25*	6.3*	4.1*	1.4	1.4	8.4
	Tolperisone	Ion channel inhibitor	74*	67*	35*	>100*	73*	49*
	PALDA	Ion channel inhibitor	5.2	13	10	6.1	15	15
	SKF 96365	Ion channel inhibitor	>100*	51*	58*	8.1	9.5	82*
	Floxuridine	Antimetabolite	0.44	0.43	0.45	0.82	0.76	1.4
	Thioguanosine	Antimetabolite	1.04	1.03	0.79	1.4	1.1	0.78
	Quinidine	NA⁺ K⁺ ATPase inhibitor	>100	>100	>100*	74*	>100	61*
	LY 171883	LTD4 antagonist	>100	>100	>100	>100	>100	>100
	Caffeic acid phenethyl ester	NFKB inhibitor	>100*	>100*	75*	21*	49*	36*
	IKK 16	IKK 16 inhibitor	3.6	4.8	6.1	5.9	6.5	6.6

Table 1. Summary of IC50 in the Alamar Blue viability assay readout for eight resupplied agonists and 13 antagonists toward the Y79 and RB355 human retinoblastoma cell lines.