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. 2013 Mar 19;8(3):e59142. doi: 10.1371/journal.pone.0059142

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© 2013 Aslanidi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Nuclear and cytoplasmic fraction of H2.35 cell infected with AAV2-WT, AAV2-Y444+500+730F and AAV2- Y444+500+730F+T491V mutant were separated and qPCR analysis was performed to evaluate vector genome distribution within cell in 16 h (a) and 48 h (b) post infection. **P<0.001 vs. WT in nucleus was considered as significant.