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. 2012 Dec 20;1(1):73. doi: 10.1186/2193-1801-1-73

Table 2.

Spectrophotometric determination of Chitinolytic and Exochitinase activity ofTrichodermaisolates in media supplemented with colloidal chitin derived fromRhizoctoniacell wall (RCW) and Commercial chitin (Cc)

Isolate No. NAGA conc. (mg/ml) Volume activity (U/ml X 10-3)
RCW Cc RCW Cc
GROUP A(T. harzianum / viride)
1 57.67 54.33 4.26 4.54
4 51 111 4.26 17.89
6 41 41 6.82 6.76
10 37.67 37.67 1.99 6.65
14 51 47.67 3.69 7.04
20 74.33 91 33.17 3.41
25 87.67 61 4.66 5.62
32 74.33 104.33 34.02 5.85
68 44.33 64.33 1.25 2.95
73 127.67 77.67 2.84 3.12
120 91 57.67 2.10 5.57
207 67.67 54.33 2.89 0.63
211 64.33 71 1.31 1.76
217 64.33 37.67 9.09 1.48
Th1 74.33 84.33 0.63 1.19
Tv(t) 71 54.33 4.89 7.1
Mean 67.46 65.58 7.37 5.09
(5.59) (5.66) (2.62) (1.02)
P 0.4837ns 0.0004s
Group B (T. citirinoviride)
24 61 57.67 13.52 20.51
27 47.67 61 1.36 2.73
28 57.67 54.33 9.26 27.83
29 67.67 141 5.96 12.49
43 57.67 54.33 14.37 3.41
Mean 58.34 73.67 8.89 13.39
(3.23) (16.88) (2.42) (4.86)
P 0.0037s 0.102ns
Group C (T. aureoviride)
12 81 54.33 0.17 18.80
38 81 114.33 22.44 1.76
48 67.67 51 35.16 27.21
55 81 61 2.49 4.88
62 71 47.67 33.06 18.69
98 101 61 17.44 1.25
107 84.33 41 8.69 8.80
202 87.67 107.67 35.78 22.15
249 81 51 13.18 0.85
297 77.67 51 3.35 21.24
231a 87.67 64.33 15.90 27.38
Gv 74.33 61 12.89 24.77
Mean 81.28 63.78 16.71 14.82
(2.51) (6.66) (3.64) (3.04)
P 0.0015s 0.2799ns
Group D (T. virens)
85 61 327.67 9.88 19.54
93 64.33 154.33 30.33 14.26
99 64.33 234.33 0.68 12.55
152 77.67 71 34.76 19.09
173 71 77.67 9.43 19.77
226 64.33 107.67 23.57 15.62
261 71 77.67 30.16 32.60
326 61 94.33 26.18 19.82
145b1 174.33 64.33 22.38 23.69
Mean 78.78 134.33 20.82 19.66
P 0.0091s 0.0282s
Group E (Isolates of Sec.L ongibrachiatum)
Cb4 84.33 81 20.85 14.88
2 91 61 5.62 16.36
46 84.33 54.33 23.69 10.51
53 84.33 51 7.61 14.77
75 81 57.67 19.31 13.69
97 74.33 64.33 2.73 1.48
102 84.33 61 35.5 15.68
Mean 83.38 61.48 16.47 12.48
(1.89) (3.67) (4.44) (1.96)
P 0.065ns 0.0342s
Group F (Isolates of Sec. Pachybasium)
8 74.33 94.33 4.71 13.41
91 47.67 51 0.45 0.91
106 67.67 54.33 1.54 2.61
111 77.67 74.33 25.28 7.78
114 71 101 1.82 8.41
204 114.33 61 12.16 5.79
238 87.67 57.67 1.02 7.89
243 91 51 24.31 13.41
332 87.67 57.67 0.34 1.42
125b 94.33 61 5.79 9.09
233b 77.67 57.67 5.62 21.87
40(1)b 74.33 47.67 13.92 9.71
Mean 80.45 64.06 8.08 8.53
(4.75) (4.95) (2.56) (1.69)
P 0.4463ns 0.0886ns

Figures in parenthesis represent standard error.

s=significant(P-value<0.05);ns= non-significant (P-value>0.05).