Table 2.
Spectrophotometric determination of Chitinolytic and Exochitinase activity ofTrichodermaisolates in media supplemented with colloidal chitin derived fromRhizoctoniacell wall (RCW) and Commercial chitin (Cc)
| Isolate No. | NAGA conc. (mg/ml) | Volume activity (U/ml X 10-3) | ||
|---|---|---|---|---|
| RCW | Cc | RCW | Cc | |
| GROUP A(T. harzianum / viride) | ||||
| 1 | 57.67 | 54.33 | 4.26 | 4.54 |
| 4 | 51 | 111 | 4.26 | 17.89 |
| 6 | 41 | 41 | 6.82 | 6.76 |
| 10 | 37.67 | 37.67 | 1.99 | 6.65 |
| 14 | 51 | 47.67 | 3.69 | 7.04 |
| 20 | 74.33 | 91 | 33.17 | 3.41 |
| 25 | 87.67 | 61 | 4.66 | 5.62 |
| 32 | 74.33 | 104.33 | 34.02 | 5.85 |
| 68 | 44.33 | 64.33 | 1.25 | 2.95 |
| 73 | 127.67 | 77.67 | 2.84 | 3.12 |
| 120 | 91 | 57.67 | 2.10 | 5.57 |
| 207 | 67.67 | 54.33 | 2.89 | 0.63 |
| 211 | 64.33 | 71 | 1.31 | 1.76 |
| 217 | 64.33 | 37.67 | 9.09 | 1.48 |
| Th1 | 74.33 | 84.33 | 0.63 | 1.19 |
| Tv(t) | 71 | 54.33 | 4.89 | 7.1 |
| Mean | 67.46 | 65.58 | 7.37 | 5.09 |
| (5.59) | (5.66) | (2.62) | (1.02) | |
| P | 0.4837ns | 0.0004s | ||
| Group B (T. citirinoviride) | ||||
| 24 | 61 | 57.67 | 13.52 | 20.51 |
| 27 | 47.67 | 61 | 1.36 | 2.73 |
| 28 | 57.67 | 54.33 | 9.26 | 27.83 |
| 29 | 67.67 | 141 | 5.96 | 12.49 |
| 43 | 57.67 | 54.33 | 14.37 | 3.41 |
| Mean | 58.34 | 73.67 | 8.89 | 13.39 |
| (3.23) | (16.88) | (2.42) | (4.86) | |
| P | 0.0037s | 0.102ns | ||
| Group C (T. aureoviride) | ||||
| 12 | 81 | 54.33 | 0.17 | 18.80 |
| 38 | 81 | 114.33 | 22.44 | 1.76 |
| 48 | 67.67 | 51 | 35.16 | 27.21 |
| 55 | 81 | 61 | 2.49 | 4.88 |
| 62 | 71 | 47.67 | 33.06 | 18.69 |
| 98 | 101 | 61 | 17.44 | 1.25 |
| 107 | 84.33 | 41 | 8.69 | 8.80 |
| 202 | 87.67 | 107.67 | 35.78 | 22.15 |
| 249 | 81 | 51 | 13.18 | 0.85 |
| 297 | 77.67 | 51 | 3.35 | 21.24 |
| 231a | 87.67 | 64.33 | 15.90 | 27.38 |
| Gv | 74.33 | 61 | 12.89 | 24.77 |
| Mean | 81.28 | 63.78 | 16.71 | 14.82 |
| (2.51) | (6.66) | (3.64) | (3.04) | |
| P | 0.0015s | 0.2799ns | ||
| Group D (T. virens) | ||||
| 85 | 61 | 327.67 | 9.88 | 19.54 |
| 93 | 64.33 | 154.33 | 30.33 | 14.26 |
| 99 | 64.33 | 234.33 | 0.68 | 12.55 |
| 152 | 77.67 | 71 | 34.76 | 19.09 |
| 173 | 71 | 77.67 | 9.43 | 19.77 |
| 226 | 64.33 | 107.67 | 23.57 | 15.62 |
| 261 | 71 | 77.67 | 30.16 | 32.60 |
| 326 | 61 | 94.33 | 26.18 | 19.82 |
| 145b1 | 174.33 | 64.33 | 22.38 | 23.69 |
| Mean | 78.78 | 134.33 | 20.82 | 19.66 |
| P | 0.0091s | 0.0282s | ||
| Group E (Isolates of Sec.L ongibrachiatum) | ||||
| Cb4 | 84.33 | 81 | 20.85 | 14.88 |
| 2 | 91 | 61 | 5.62 | 16.36 |
| 46 | 84.33 | 54.33 | 23.69 | 10.51 |
| 53 | 84.33 | 51 | 7.61 | 14.77 |
| 75 | 81 | 57.67 | 19.31 | 13.69 |
| 97 | 74.33 | 64.33 | 2.73 | 1.48 |
| 102 | 84.33 | 61 | 35.5 | 15.68 |
| Mean | 83.38 | 61.48 | 16.47 | 12.48 |
| (1.89) | (3.67) | (4.44) | (1.96) | |
| P | 0.065ns | 0.0342s | ||
| Group F (Isolates of Sec. Pachybasium) | ||||
| 8 | 74.33 | 94.33 | 4.71 | 13.41 |
| 91 | 47.67 | 51 | 0.45 | 0.91 |
| 106 | 67.67 | 54.33 | 1.54 | 2.61 |
| 111 | 77.67 | 74.33 | 25.28 | 7.78 |
| 114 | 71 | 101 | 1.82 | 8.41 |
| 204 | 114.33 | 61 | 12.16 | 5.79 |
| 238 | 87.67 | 57.67 | 1.02 | 7.89 |
| 243 | 91 | 51 | 24.31 | 13.41 |
| 332 | 87.67 | 57.67 | 0.34 | 1.42 |
| 125b | 94.33 | 61 | 5.79 | 9.09 |
| 233b | 77.67 | 57.67 | 5.62 | 21.87 |
| 40(1)b | 74.33 | 47.67 | 13.92 | 9.71 |
| Mean | 80.45 | 64.06 | 8.08 | 8.53 |
| (4.75) | (4.95) | (2.56) | (1.69) | |
| P | 0.4463ns | 0.0886ns | ||
Figures in parenthesis represent standard error.
s=significant(P-value<0.05);ns= non-significant (P-value>0.05).