Fig. 2.

Correlation between membrane depolarisation and killing of target bacteria by PXL150. a The fluorescence intensity and bacterial viable counts were monitored at indicated time points after the addition of 5 μg/ml PXL150 to a bacterial suspension supplemented with 100 mM KCl and 0.4 mM DiSC3(5). The maximum polarisation intensity of 5 μg/ml PXL150 or bacterial counts at time 0 was set to 100 %. b The fluorescence intensity and bacterial viable counts were monitored at 1.5 min post-addition of PXL150 in a concentration range of 0.625 to 160 μg/ml to a bacterial suspension supplemented with 100 mM KCl and 0.4 mM DiSC3(5). The maximum fluorescence intensity of 160 μg/ml PXL150 at the 1.5-min time point or bacterial counts without peptide added was set to 100 %