Figure 4.

Effect of 30 or 60 minutes of trauma alone or combined trauma and hemorrhage on GH-induced STAT5 phosphorylation. Mice were subjected to trauma alone and injected with GH or saline immediately (T0′), 30 or 60 minutes later (T30′ and T60′), or after hemorrhage for 30 or 60 minutes (TH30′ and TH60′). A and C, Western analysis of liver lysates was performed, and blots were probed with specific anti-PY694/699-STAT5 and anti-STAT5 antibodies. Representative lanes from the scanned image of a single film were chosen; bands of interest were cropped and rearranged to enhance clarity. B and D. Bands of interest were quantified by scanning densitometry, and differences in STAT5 phosphorylation between GH+ groups were determined. Data are presented as mean ± SEM percent change in STAT5 phosphorylation, normalized to total STAT5. E, Comparison of GH-induced STAT5 phosphorylation after acute injury (compiling information from Figures 3 and 4). Data are expressed as mean ± SEM percent change in STAT5 phosphorylation. P-STAT5 after T0′ was arbitrarily set to 100%, and P-STAT5 after trauma alone and combined trauma and hemorrhage for 30, 60, and 90 minutes are expressed relative to T0′. **P < .01 vs T0′; ***P < .001 vs T0′; %P < .05 vs T30′; ^^P < .01 vs T60′, all via 1-way ANOVA; $P < .05 vs T90′ via two-tailed, Welch-corrected t test; NS, no statistically significant difference vs T0′; n = 6 or 7 mice per group. T, total.