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Substrate specificity of Amy13A
| Substrate | Specific activity (U/mg) |
|---|---|
| Soluble starch | 1,000 |
| Corn starch | 850 |
| Potato starch | 790 |
| Rice starch | 340 |
| Amylose | 480 |
| Amylopectin from corn | 330 |
| Amylopectin from potato | 70 |
| Pullulan | N.D. |
| α-Cyclodextrin | N.D. |
| β-Cyclodextrin | N.D. |
| γ-Cyclodextrin | N.D. |
For the determination of substrate specificity, recombinant enzyme was incubated for 5 min in Tris–HCl buffer (50 mM, pH 7.0) at 80 °C with different substrates (0.5 % w/v)
