Rat preglomerular vascular smooth muscle cells were incubated for 1 h without or with guanosine (30 μmol/l) and without or with adenosine (A), uridine (B), thymidine (C), or cytidine (D) (each at 3 μmol/l). Medium was assayed for adenosine, uridine, thymidine, and cytidine by mass spectrometry. Values represent means ± SE. <DL, less than detection limit. aSignificantly different from all other groups in panel.