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The main steps in the preparation of brain slices. A. Schematic representation of the cutting directions. B. Brain glued to the slicing platform in a coronal position. C. Brain slices transferred into the 80 mm diameter, flat-bottomed glass beaker. D. Beaker covered by custom-fabricated lid composed of a Teflon fluorinated ethylene-propylene film. E. Setup for the incubation-equilibration period.
