Table 1.

Composition of the working aECF solution

	Weight (g)/volume (ml)
Glucose	1.802 g
Milli-Q water*	600 ml
Stock aECF	100 ml
280 mM CaCl2	5 ml
400 mM Ascorbic acid	1 ml
pH (23°C)	Adjust pH to 7.6 (up to 1.5 ml of 10 M NaOH)
Milli-Q water	Adjust volume to 1000 ml

* should be dispensed the day before the experiment.