Table 4.

Preparation of samples for assessment of viability of brain slices based on the activity of released lactate dehydrogenase

	aECF buffer	Low control	Sample	High control	Reaction mixture*
Background	100 μl				100 μl
Low control		100 μl			100 μl
Sample			100 μl		100 μl
High control				100 μl	100 μl

* The reaction mixture should be prepared ex tempore according to the manufacturer's instructions.