Table 5.
Checklist before starting the experiments
|
aECF |
Stock aECF |
| |
280 mM CaCl2 |
| |
400 mM ascorbic acid |
| |
Dispensed the day before 1 L MQ water |
| |
Oxygen supply |
| |
pH meter |
|
Apparatus |
Orbital shaking incubator |
| |
Vibratome |
| |
Nitrogen evaporator |
| |
Ultrasonic bath |
| |
Ultrasonic processor |
| |
Centrifuge |
| |
Water bath |
| |
ELISA reader |
|
Glass- and lab-ware |
Petri dishes |
| |
45 mm high, 80 mm diameter, flat-bottomed glass beakers |
| |
Custom-fabricated lids of Teflon FEP film for beakers |
| |
Scintillation vials |
| |
Surgical instruments for dissection of brain |
| |
Ice buckets |
| |
Pre-labeled Eppendorf tubes |
| |
Nunc 96-well plates |
| |
Blank brain homogenate (in 4 volumes (w/v) of aECF) |
|
Miscellaneous |
Cyanoacrylate glue |
| Cytotoxicity detection kit |