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. 2012 Dec 18;304(5):E486–E494. doi: 10.1152/ajpendo.00519.2012

Table 1.

Primer sequences for gene expression

Genes (bp) Gene ID Primer Sequence (5′→3′) Size of PCR Product
Pomc NM 139326 Forward: 5′-TCCATAGACGTGTGGAGCTG-3′ 174
Reverse: 5′-ACGTACTTCCGGGGATTTTC-3′
Npy NM 012614 Forward: 5′-AGAGATCCAGCCCTGAGACA-3′ 236
Reverse: 5′-AACGACAACAAGGGAAATGG-3′
Lepr NM 012596 Forward: 5′-CCTGAAACATTTGAGCATCTTT-3′ 370
Reverse: 5′-CGATGCACTGGCTGACAGAA-3′
Stat3 NM 012747 Forward: 5′-TGATGCGCTCTTATGTGAGG-3′ 104
Reverse: 5′-GGCGGACAGAACATAGGTGT-3′
Socs3 NM 053565 Forward: 5′-TTGTCGGAAGACTGTCAACG-3′ 183
Reverse: 5′-GGCTGGATTTTTGTGCTTGT-3′

Pomc, proopiomelanocortin; Npy, neuropeptide Y; Lepr, long form of leptin receptor; Stat3, signal transducer and activator of transcription 3; Socs3, suppressor of cytokine signaling 3.