Fig. 4.

Chymase-mediated intestinal epithelial permeability is attenuated by MMP-2 inhibition. A: TER of Caco-2 BBe cells was stimulated basolaterally with ARP100 (5 μg/ml) in vehicle-PBS (control) or chymase (0.05 U/ml) in the absence or presence of ARP100 (5 μg/ml). Values are expressed as percent TER change from time 0. B and C: gelatin zymography of basolateral supernatant and densitometry of MMP-2 activity of Caco-2 BBe cells stimulated basolaterally with ARP100 (5 μg/ml) in vehicle-PBS or chymase (0.05 U/ml) in the absence or presence of the MMP-2 inhibitor ARP100 (5 mg/ml). Values are means ± SE; n = 4 individual wells per data point. *P < 0.01; ***P < 0.005.