Fig. 6.

NPY does not alter pro-TRH processing enzymes in the PVN. A: simplified model of the pro-TRH processing cascade is depicted and has been described in the rat PVN. One copy of pro-TRH contains 5 copies of TRH. Black bars represent TRH. B–F: male rats received intra-PVN infusion with 5 μg NPY, and 60 min later, food intake was measured and PVN samples collected for Western blot analyses. B: food intake (g; n = 4; t-test, P = 0.0098) C: PC1 protein levels (n = 4; t-test, P = 0.80). D: PC2 protein levels (n = 4; t-test P = 0.047). E: CPE protein levels (n = 4; t-test, P = 0.78). F: CPD protein levels (n = 4; t-test, P = 0.79). Data are means ± SE. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control.