Fig. 3.

GLUT3 mRNA expression studies. Representative Northern blots in the presence (+) and absence (−) of glucose (5 mM; n = 2/time point/condition) with ribosomal S2 mRNA as the internal control (A, bottom panel) and reverse transcription-quantitative PCR (n = 5–6/time point/condition; B) demonstrate GLUT3 mRNA (A, top) in N2A cells exposed to Nx and Hx in a time-dependent manner. *P < 0.01 vs. 24-h Nx; †P < 0.001 vs. 48-h Nx; #P < 0.01 vs. 24-h Hx. Actinomycin D (Act D; 7 μg/ml; C and E) or cycloheximide (Cyx; 20 μg/ml; D and E) treatment over 0, 1.5, 3, and 6 h following 24 h of Nx or Hx (C and D) or pretreatment of N2A cells before exposure to Nx or Hx for 0 [control (con)], 7, and 24 h (E). Blots in C, D, and E, top, demonstrate the corresponding representative Northern blots (top blots), with ribosomal S2 mRNA (bottom blots) serving as the internal control. The results are depicted as a percent of the 0-h Nx (or con) value (C and D: n = 4/time point/condition/treatment, *P < 0.05 vs. the 0 time value in the same condition; E: n = 2/time point/condition/treatment, *P < 0.002 vs. the cycloheximide Nx counterpart and +P < 0.005 vs. the respective con for Nx or Hx).