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Stable isotope tracer protocol. Unlabeled l-phenylalanine (Phe) was injected at −5 min, and l-[ring-13C6]Phe and l-[U-13C4,15N]threonine (Thr) were injected at 0 min. Muscle biopsies were obtained at baseline and at 5, 30, and 60 min. Blood was drawn at baseline and at 5, 15, 30, 45, and 60 min.
