Fig. 2.

Regulation of APJ in kidneys from diabetic mice. A: renal expression of APJ was studied by immunohistochemistry on sections of paraffin-embedded kidneys from 3-mo-old mice. Arrows point to glomeruli. Top, middle, and bottom: represent 3 different mice. B: APJ protein was measured by immunoblot on kidney cortex homogenates from 3-mo-old mice. Bottom: combined data obtained on kidneys from 5 individual mice for each experimental condition. C: APJ mRNA was measured by RT-quantitative (q)PCR on total RNA extracted from kidney cortex and normalized using GAPDH expression; n = 5 mice per group. Numbers inside brackets represent P values calculated by ANOVA; con, control.