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Effect of apelin on glomerular hypertrophy. A: renal histology was studied by periodic acid Schiff staining on section of paraffin-embedded kidneys. B: glomerular tuft area was traced and measured using the Image Pro Plus software. A minimum of 30 glomeruli per mouse were counted; n = 5 mice per group. Numbers inside brackets represent P values calculated by ANOVA; ns, not significant.
