Fig. 5.

Effect of apelin on albuminuria. A: 24-h urine collection was performed on the eve of death; n = 5 mice per group. B: podocytes were identified as WT1⁺ nuclei in the glomeruli. WT1 was detected by immunohistochemistry on sections of frozen kidneys from 6-mo-old mice. Top, middle, and bottom: represent 3 different mice. C: WT1⁺ nuclei were counted by a blinded investigator. Numbers inside brackets represent P values calculated by ANOVA; ns, not significant. Megalin expression was assessed by immunohistochemistry on sections from paraffin-embedded kidneys (D) and by immunoblot on kidney cortex homogenates (E) from 6-mo-old mice; n = 5 mice per group.