Fig. 7.

Effect of apelin on mediators of renal inflammation. Monocyte attractant protein 1 (MCP1; A) and vascular cell adhesion molecule 1 (VCAM1; D) were detected by immunohistochemistry on sections of paraffin-embedded kidneys from 6-mo-old mice. Top, middle, and bottom: represent 3 different mice. MCP1 protein (B) and VCAM1 protein (E) was measured by immunoblot on kidney cortex homogenates from 6-mo-old mice. Bottom: combined data obtained on kidneys from 5 individual mice for each experimental condition. MCP1 mRNA (C) and VCAM1 mRNA (F) were measured by RT-qPCR on total RNA extracted from kidney cortex and normalized using GAPDH expression; n = 5 mice per group. Numbers inside brackets represent P values calculated by ANOVA.