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Effect of apelin on NF-κB activation. A: nuclear translocation of the p65 subunit of NF-κB was assessed by immunoblot on nuclear fractions purified from kidney cortex from 6-mo-old mice. Loading was assessed by measuring expression of lamin B1. B: ratio of p65/lamin B1 in nuclear extracts. C: ratio of p-p65/p65 in nuclear extracts. D: binding of p65 to VCAM1 promoter by ChIP; n = 5 mice per group. Numbers inside brackets represent P values calculated by ANOVA.
