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Effect of apelin on catalase expression. A: catalase protein was detected by immunoblotting on kidney cortex homogenates from 6-mo-old mice. Bottom: densitometric analysis of catalase expression, normalized for loading using actin. B: catalase mRNA was measured by RT-qPCR on total RNA extracted from kidney cortex and normalized using GAPDH expression; n = 5 mice per group. Numbers inside brackets represent P values calculated by ANOVA.
