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. 2013 Jan 18;304(6):L445–L455. doi: 10.1152/ajplung.00261.2012

Fig. 1.

Fig. 1.

The effect of lipopolysaccharide (LPS) on 2-OH-E+ formation and intercellular adhesion molecule (ICAM)-1 expression in human pulmonary microvascular endothelial cells (HPMEC). A: 2-OH-E+ peaks (pM/mg protein) were measured by HPLC to quantify intracellular superoxide formation. A representative chromatogram showing LPS-treated and control cells is shown. B: cumulative data from 3 experiments showing increased 2-OH-E+ formation with LPS treatment at 1 h. *P = 0.006 (control vs. LPS-treated), n = 3. C: ICAM-1 mRNA expression was quantified in whole cell lysates by real-time PCR at 4 and 24 h. #P = 0.03 (control vs. 4 h LPS- treated); ##P = 0.002 (control vs. 24 h LPS-treated), n = 4. D: representative blot showing changes in ICAM-1 expression after LPS treatment for 12 h. E: densitometric quantification of ICAM-1 expression after 12 and 24 h LPS treatment. $P = 0.001 (control vs. 12 h LPS); $$P = 0.001 (control vs. 24 h LPS), n = 4.