Fig. 3.

Effect of LPS on p67phox intracellular compartmentalization. A: whole cell (WC) and membrane (Memb) fractions obtained from control and LPS-treated HPMEC by differential buffer extraction were immunoblotted for p67phox and Noxa1. Image represented originally contained nuclear fractions that are not shown. B: p67phox expression normalized to Grp94 was quantified by densitometry to show the increase in p67phox in the membrane fraction with LPS. $P = 0.02 (control vs. 15 min LPS); $$P = 0.004 (control vs. 30 min LPS), n = 4.