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. 2012 Dec 21;304(5):H687–H696. doi: 10.1152/ajpheart.00744.2012

Fig. 3.

Fig. 3.

VEGF mobilizes the nonraft pool of VEGFR2 into Cav1-enriched membrane raft fractions. HUVECs cultured overnight in serum-depleted medium were either mock treated or treated with VEGF-A (50 ng/ml) for 30 min; cell homogenates were fractionated using OptiPrep gradient centrifugation and analyzed via immunoblotting using the indicated Abs. A and C: representative blots indicate the fractionation profiles of VEGFR2, Myo1c, and Cav1 with respect to markers for PM (syntaxin 4), endosomes (EEA1), Golgi (TGN46) in serum-starved cells and VEGF-stimulated cells, respectively. B and D: densitometric quantification of bands in each OptiPrep gradient fraction is represented as the percentage of total VEGFR2, Myo1c, or Cav1 in each fraction of serum-starved cells, either untreated or treated with VEGF. The percentage given is the mean ± SD for n = 3 experiments.