Table 3.
Permeability ratios PX/PNa of monovalent cations for Q117 mutants
Ion X+ | WT ChR2 | C34A/C36A | Q117E | Q117R | Q117V | Q117Y |
---|---|---|---|---|---|---|
Li+ | 1.03 (9) | 1.07 (5) | 0.91 (6) | 0.96 (6) | 1.02 (7) | 1.13 (10) |
Na+ | 1.00 (11) | 0.97 (8) | 0.52 (19) | 0.95 (7) | 0.98 (7) | 1.00 (13) |
K+ | 0.81 (14) | 0.77 (7) | 0.48 (8) | 0.47 (5) | 0.59 (13) | 0.89 (21) |
Rb+ | 0.45 (5) | 0.50 (5) | 0.35 (6) | 0.48 (5) | 0.45 (5) | 0.54 (10) |
Cs+ | 0.47 (5) | 0.45 (5) | 0.36 (6) | 0.44 (5) | 0.45 (8) | 0.49 (8) |
Permeability ratios determined as described in Materials and Methods. [X+] = 115 mM, pH = 9. Mean values ± SE of the mean (number of experiments).