Figure 1.

High-time-resolution PFIs. (A) Sixteen PFIs are summed into one total intensity with photons binned within each 0.8 ms cycle (blue and gray) or averaged over 10 cycles (black) that is relatively constant before photobleaching to background intensity at ∼1.5 s. (B and C–F) The total intensity (B) and the 16 PFIs (C–F) comprising the interval before photobleaching (blue region in panel A) are shown expanded, with intensities averaged over 10 cycles. The individual measured intensities depend on the relative orientation between the fluorophore dipole axis and the direction of the input laser polarizations p (blue), s (red), L (green), and R (magenta), and the polarized fluorescence detected on APDx (C and D) and APDy (E and F) for beams 1 and 2, respectively (see Fig. S1 for details on the experimental setup). (G) To illustrate the high time resolution of each PFI, the photons collected during each 0.8 ms cycle of the laser polarizations are shown for _p2I_x (blue) and _s2I_x (red) binned at 0.8 ms. The MCCP algorithm gives the times of intensity change points (vertical black dashed lines) along with a 95% CI (gray area). The average intensity between neighboring change points (horizontal lines) is also shown in B–G.