Fig. 6.

Losartan reduced distal limb perfusion in Cx40^−/− (40^−/−) mice. A: distal limb perfusion was modestly diminished in losartan (Los)-treated (n = 15) vs. untreated (n = 12) Cx40^−/− male mice; however, this difference did not achieve significance (P = 0.07) due to offsetting effects of reduced blood flow (B; *P < 0.02) and the small increase in perfused sites (C; P = 0.09). D and E: microvascular density in losartan-treated (n = 4 males) and untreated (n = 5 males) Cx40^−/− mice, quantified in cross-sections of gastrocnemius muscle from the nonsurgical limb (scale bar = 100 μm), was not significantly different. F: angiogram of the gracilis region of the nonsurgical hindlimb of a losartan-treated Cx40^−/− mouse. Scale bar = 1 mm. G: no differences between treated and untreated Cx40^−/− mice were detected for hindlimb vessel diameter (P = 0.64). Arrowhead on y-axis indicates the mean value observed for the nonsurgical limbs of male WT mice.