Fig. 9.

Macrophage infiltration of the ischemic hindlimbs of Cx40^−/− mice was diminished by losartan (Los) treatment. A: representative images of gastrocnemius muscle (in cross section, stained to reveal F4/80 antigen) isolated 14 days after severe surgery from the surgical limbs of untreated (top) or losartan-treated (bottom) Cx40^−/− mice. Note that macrophage staining (dark brown) was more extensive in sections from untreated vs. treated mice (scale bar = 100 μm). B: area of the surgical limb vs. nonsurgical limb infiltrated by macrophages was significantly (*P < 0.05) greater in untreated than in treated Cx40^−/− mice (n = 6 for both groups).