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. 2013 Mar 19;104(6):1296–1303. doi: 10.1016/j.bpj.2013.01.041

Figure 2.

Figure 2

The dependence of the dissociation rate constant, kd, on oligonucleotide concentration is shown for: 80 mM NaCl, pH 7.2, no osmolyte (▴, ★), and for 180 mM NaCl, 2.5 osmolal betaine glycine, pH 7.2 (●). The specific sequence oligonucleotide was used in these experiments, except for ★, which had 0.5 μM specific sequence oligonucleotide and 4.5 μM nonspecific sequence oligonucleotide. The competitor DNA dependence seen at 80 mM NaCl is absent at the higher salt concentration. All samples contained 20 mM Imidazole, 1 mM EDTA, 2 mM DTT, and 100 μg/ ml BSA in addition to the NaCl and the osmolyte betaine glycine.