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. 2012 Aug 28;6(2):483–502. doi: 10.1093/mp/sss075

Figure 5.

Figure 5.

Effect of NtWLIM2 Expression on Arabidopsis H4A748 Gene Promoter Activation in Arabidopsis and Tobacco Protoplasts. (A) Reporter and effector constructs used in the study. In the H4 reporter construct, firefly luciferase (LUC) expression was controlled by a 604-bp-long fragment of the Arabidopsis histone H4A748 gene promoter. Effector constructs allowed cDNA expression of either full-length NtWLIM2, NtWLIM1, or GFP under control of the CaMV 35S promoter (35S). (B) NtWLIM2 activates pH4A748-controlled LUC expression in protoplasts. Effector and reporter constructs were co-transfected into Arabidopsis or tobacco protoplasts. LUC activity in protoplast extracts was measured 20h after transfection and normalized against GUS activity. The signal of reporter activity in the presence of each effector is given relative to the signal measured with the same concentrations of an empty vector, which is set to 100% (NtWLIM2, NtWLIM1, GFP: black, light gray, white bars in Arabidopsis protoplasts). Additional experiments were conducted for the NtWLIM2 effector construct in tobacco protoplasts (dark gray bars). Data represent mean values of several independent transfections (n≥3) and error bars indicate standard deviations.