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. Author manuscript; available in PMC: 2013 Mar 20.
Published in final edited form as: Cell Physiol Biochem. 2012 Jun 8;30(1):33–48. doi: 10.1159/000339047

Figure 3. Blocking NKCC1 activity accelerates AVD after TMZ treatment.

Figure 3

A. Time-lapse DIC images were used to measure changes in cell volume in GSC (#22). Cultures were treated with TMZ (500 µM) or TMZ + BMT (10 µM) and representative images of cells at times 0, 4, 8, and 12 h are shown. Inhibition of NKCC1 with BMT accelerated AVD, as evident by the loss of processes, and cell body shrinkage (arrowhead). B. Summary data of relative cell volume changes. Data are means ± SEM. n = 8–9. * p < 0.05 vs. corresponding TMZ. C. GC (#22) were exposed to normal medium (Con), Con + 10 µM BMT, 500 µM TMZ, 500 µM TMZ + 10 µM BMT or 4 µM STS for 4 h. Cell size was examined by flow cytometry using a FSC versus SSC 3D plot. 4 µM STS-treated GC were used as a positive control for AVD. Red circle: shrunken population of cells in the 3D plots. Data are means ± SD. n = 3. * p < 0.05. vs. Con.