Table 1.

Results of automated segmentation performance on 21 test images.

Stain	ID/magnification	Manual quantification (cells)	Automated quantification (cells)	Accuracy (%)	Precision (%)	Sensitivity (%)	Specificity (%)
SMActin	Day 5–40x	101	100	92.96	91	90.01	94.67
	Day 5–60x	59	60	91.82	88.33	89.83	93
	Day 7–40x	166	187	93.55	85.56	96.38	92.19
	Day 7–60x	87	94	93.96	89.24	95.40	93.10
	Day 14–40x	85	88	92.75	89.77	92.94	92.62
H&E	Day 5–40x	86	86	100	100	100	100
	Day 7–40x	141	138	99.05	100	97.87	100
	Day 7–60x	66	71	95.10	90.14	96.96	94.06
	Day 14–40x	105	108	98.69	97.22	100	97.6
	Day 14–60x	42	38	95.28	100	88.095	100
CD-31	Day 5–40x	76	77	99.38	98.70	100	98.85
	Day 5–60x	52	49	97.43	100	94.23	100
	Day 7–40x	140	188	94.32	77.77	97.22	93.66
	Day 7–60x	61	59	97.61	100	95.08	100
	Day 14–40x	98	104	97.97	94.23	100	97.97
	Day 14–60x	67	67	100	100	100	100
CD-68	Day 5–40x	99	96	91.36	76.19	96.96	89.39
	Day 5–60x	68	66	95.13	84.61	97.05	94.54
	Day 14–40x	143	138	98.55	100	96.50	100
	Day 14–60x	69	70	99.49	98.57	100	99.23
Total		1811	1884
Average				95.55	91.27	96.45	95.07