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. Author manuscript; available in PMC: 2013 Mar 20.
Published in final edited form as: Exp Brain Res. 2009 Jun 17;196(4):545–556. doi: 10.1007/s00221-009-1885-3

Figure 6.

Figure 6

CART peptide-immunoreactive neurons and axonal networks in the medulla oblongata of a LETO (A, C) and an OLETF (B, D) rat. A and B: The medial part of nucleus of solitary tract (NTS, m) contains dense immunoreactive fiber networks, whereas the lateral part (NTS, l) exhibits lower density of immunoreactive axons and scattered neurons in both LETO (A) and OLETF (B) rats. In the medial nucleus of solitary tract, fibers reveal stronger CART immunoreactivity in LETO than in OLETF rat. In nucleus ambiguous (AMB) and in inferior olive (IO) immunoreactive fibers are detected, and CART peptide containing cells are visible in region of C1 in both lean (A) and obese (B) animals. C and D: Similarly to the rostral level of medulla oblongata, the medial part of nucleus of solitary tract expresses a dense network of immunopositive fibers, while the lateral part contains immunoreactive neurons and moderate densitiy of axons in both strains. In addition, area postrema (AP) exhibits strong CART peptide immunoreactivity. Nuclei raphe obscurus (ROb), ambiguous and oliva inferior express also weak immunopositivity in both LETO (C) and OLETF (D) rats. E and F: Large magnification of the rostral portion of the medial part of nucleus of solitary tract reveals slightly denser fiber plexus in LETO than found in OLETF. Arrows point to CART-immunoreactive neuronal cell bodies. Around the nucleus of the solitary tract strongly CART-immunostained axons (arrowheads) are also visible. Antero-posterior coordinates are represented in mm distance from the Bregma (Br). Additional abbreviation: py, pyramidal tract. Scale bars = 500 μm in A–D.