Figure 4.

Time-lapse fluorescence images at selected time points. (b) Six time-lapse Mito-R, YO-PRO-1 images of normal RBA1 astrocytes. RBA-1 astrocytes become permanent to YO-PRO-1 after treatment with 20 mM H₂O₂ at 150 min. (d) Five time-lapse Mito-R, YO-PRO-1 images of ρ ⁰ RBA1 astrocytes. ρ ⁰ RBA-1 astrocytes become permanent to YO-PRO-1 after treatment with 20 mM H₂O₂ at 130 min. (a) Phase-contrast images of normal RBA1 astrocytes in six time-lapse series. (c) Phase-contrast images of ρ ⁰ RBA1 astrocytes in five time-lapse series. Images were taken at rest and after exposure of H₂O₂ at 0, 10, 30, 60, 70, 130, and 150 min. Bar =10 μm.