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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Pain. 2012 Mar 17;153(5):1091–1106. doi: 10.1016/j.pain.2012.02.015

Figure 4.

Figure 4

Immunofluorescent intensity quantification of the spinal cord dorsal horn reveals i.t. AM1710 reduces the expression of the endocannabinoid degragative enzyme, MAGL. A, B, No changes in expression of FAAH IR between non-neuropathic sham and neuropathic CCI rats following i.t. AM1710 or equivolume vehicle were observed (ANOVA, F(3,11)=1.967; p=0.1976; ANOVA, F(3,11)=3.068; p=0.0910, respectively) C, D, Compared to non-neuropathic sham operated rats given i.t. AM1710 or equivolume vehicle, neuropathic rats given i.t. vehicle showed a robust bilateral increase in dorsal horn MAGL IR. In contrast, an i.t. AM1710 injection robustly suppressed bilateral increases in dorsal spinal MAGL IR (ANOVA, F(3,11)=11.38; p=0.0029, ANOVA, F(3,11)=5.444; p=0.00247, respectively). Inset, C, non-neuropathic sham rats given i.t. AM1710 as well as neuropathic rats given i.t. vehicle showed an increase in meningeal MAGL IR compared to non-neuropathic rats given i.t. vehicle (ANOVA, F(3,11)=8.153; p=0.0081). E, F, G, H, Representative spectrally unmixed images at 20x magnification of MAGL fluorescent staining (green), and DAPI nuclear stain (blue). In all images the scale bar is equal to 50 μm.