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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Pain. 2012 Mar 17;153(5):1091–1106. doi: 10.1016/j.pain.2012.02.015

Figure 7.

Figure 7

AM1710 pre-treatment blocks gp120-induced allodynia and IL-1β cytokine production. A total of 24 animals were used for both the behavioral experiment reported here and tissues from these animals were analyzed in the reported ELISA experiments. A, B, Prior to surgical manipulation, all groups exhibited similar bilateral (ipsilateral and contralateral) BL thresholds (ANOVA, F(3,23) =1.781; p=0.1833; F(3,23) =2.311; p=0.1072, respectively). Following indwelling catheter implantation, there was no major effect of surgery on Day 6 after surgery thresholds when compared to BL thresholds or following the i.t. pretreatment of either AM1710 or vehicle (ANOVA, F(6,60) = 0.9381; p=0.1311; ANOVA, F(6,60)=2.415 ; p=0.1648, respectively). Animals given i.t. gp120 developed strong allodynia in both left and right paws at 20 min compared to pre-gp120 threshold values (ANOVA, F(3,20)=61.72; p<0.0001 ANOVA, F(3,20)=75.73; p<0.0001, respectively). C, Quantification of IL-1β protein by ELISA revealed IL-1β was significantly increased in left DRG of allodynic rats following i.t. gp120 given a pretreatment with i.t. vehicle of AM1710 (ANOVA, F(3,13)= 7.785; p=0.0057). D, a trend in the levels of TNF-α protein, although not statistically significant (ANOVA, F(3,14) = 2.977; p=0.0741).