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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: J Immunol. 2012 Jul 9;189(4):1585–1593. doi: 10.4049/jimmunol.1200342

Figure 8. Tregs can control pDC activation.

Figure 8

Tregs were tested for their ability to control DC activation. 2×106 in-vitro expanded Tregs were injected (i.p) on day 8 and 12 into recipient mice. Collagenase-treated splenocytes from 16-day old WT and IL-2-KO mice were stained and analyzed by flow cytometry. FACS profiles of pDCs (top panel) were gated on Thy1.2−CD11clo B220+PDCA-1+ cells and cDCs (bottom panel) were gated on Thy1.2−CD11chiB220− cells then surface marker expression analyzed by mean fluorescent intensity (MFI). Dots represent the MFI from three individual mice in three experiments.