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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: Circ Res. 2013 Jan 2;112(4):606–617. doi: 10.1161/CIRCRESAHA.112.300603

Figure 4. Mat2aa regulates cardiomyocyte cell size and cell number.

Figure 4

(A) Representative images of embryonic hearts at 3 dpf after co-staining with anti-β-catenin (green) and anti-Mef2 (red) antibodies from a GBT0364 WT sibling and homozygous mutant with or without injection of 1 nL of p53_MO. Scale bar=20 μm. (B) Quantification of cardiomyocyte (CM) cell size. Injection of p53_MO did not alter the reduced CM size in GBT0364 homozygous mutants. (C) Quantification of CM cell circularity. No significant changes of circularity were observed. (D) Representative images of embryonic hearts at 3 dpf after co-staining with anti-TUNEL (green) and anti-Mef2 (red) antibodies from a GBT0364 WT sibling and homozygous mutant with or without injection of 1 nL p53_MO. (E) Quantification of CM cell number in the ventricle. Injection of p53_MO partially rescued the reduced CM number in GBT0364 homozygous mutant embryos. (F) Quantification of the CM cell apoptosis index in the ventricle. Injection of p53_MO partially rescued the increased TUNEL index of CMs in GBT0364 homozygous mutant embryos. (G) Representative images of embryonic hearts at 3 dpf after co-staining with anti-PCNA (green) and anti-Mef2 (red) antibodies from a GBT0364 WT sibling and homozygous mutants with or without injection of 1 nL p53_MO. Arrows indicate PCNA+/Mef2+ cells. (H) Quantification of the CM cell proliferation index in the ventricle. Injection of p53_MO did not alter the reduced PCNA index of CMs in GBT0364 homozygous mutant embryos. n=6, *P<0.05.