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. Author manuscript; available in PMC: 2013 Mar 20.
Published in final edited form as: J Immunol. 2009 Sep 4;183(7):4415–4421. doi: 10.4049/jimmunol.0901021

FIGURE 2.

FIGURE 2

Differing stimulatory activities of GCK127 and GCK152 in the context of human vs mouse CD1d molecules against human vs murine iNKT cells in vitro. In A and B, 2 × 104 human iNKT cells or mouse iNKT cells were cocultured with 2 × 104 Hela-hCD1d or A20-mCD1d, respectively, in the presence of various concentrations of each glycolipid, ranging from 0.01 to 1 μg/ml in a 96-well culture plate. In C and D, 1 × 105 human iNKT cells or mouse iNKT cells were cocultured with 1 × 105 of A20-mCD1d or Hela-hCD1d, respectively, in the presence of various concentrations of each glycolipid, ranging from 0.01 to 1 μg/ml in a 96-well culture plate. After 24 h of incubation, supernatants were collected, and the concentration of hIFN-γ and hIL-4, or mIFN-γ and mIL-4, was determined by ELISA. Results represent one of three similar experiments.