Skip to main content
. Author manuscript; available in PMC: 2013 Mar 20.
Published in final edited form as: J Immunol. 2008 Jul 1;181(1):574–585. doi: 10.4049/jimmunol.181.1.574

FIGURE 1.

FIGURE 1

The DLN is an early site of IgA production following VRP infection. Groups of BALB/c mice were immunized in the rear footpad with HA-VRP (1 × 105 IU), I-Flu alone (10 μg), or I-Flu (10 μg) plus GFP-VRP (1 × 105 IU) as an adjuvant at weeks 0 and 4. At the indicated time points, lymphoid organ cultures were established from the spleen (A and B), nasal epithelium (C and D), and DLN (E and F) and evaluated for the presence of flu-specific IgG (A, C, and E) and IgA Abs (B, D, and F) by ELISA. Values represent mean ± SEM. *, p < 0.05; **, p < 0.01; and ***, p < 0.001 compared with I-Flu alone, as determined by ANOVA.